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These conditions. Moderately Stabilized 32 Mutants in Vivo Show Intermediate Affinity for Chaperones--To clarify the relationship between the in vivo stability of 32 and the affinity for chaperones, we examined two other 32 mutants, A50S and K51E, that exhibit moderately increased in vivo stability (half-life approximately 4 times longer than that of wild type) (26). The purified 32 mutants were c
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I54A A50S K51Ea b1.6 m2.4 m2.4 m14.7 47.4.1 40.10.8 62.3 40.4 59.9.2 38.6 26.5 18.Data from the experiment shown in Fig. 4, B and C. Data from the experiment shown in Fig. 5, B and C.FIGURE 6. Correlation between the percentage of 32 that remains free in gel filtration analysis and the stability of 32 (half-life) in vivo. Gel filtration experiments were repeated 2?4 times, and the percentages of f
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